nextgen sequencing method  (NextGen Sciences)

Journal: Analytical and Bioanalytical Chemistry

Article Title: Robust discrimination between closely related species of salmon based on DNA fragments

doi: 10.1007/s00216-024-05724-9

Figure Lengend Snippet: Sequence of measured nucleotides in a fragment of mtDNA of NIST RM 8256 (A \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$=$$\end{document} = adenine, C \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$=$$\end{document} = cytosine, G \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$=$$\end{document} = guanine, T \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$=$$\end{document} = thymine), and ASCII codes representing the corresponding Phred quality scores

Article Snippet: We have developed a novel method to quantify the confidence surrounding the ability of a representative NextGen Sequencing method to verify the genetic identity of RM 8256, Wild-caught Coho Salmon [ ], which is a fresh frozen fish homogenate prepared from multiple individuals collected off the coast of Alaska, USA.

Techniques: Sequencing

Journal: Analytical and Bioanalytical Chemistry

Article Title: Robust discrimination between closely related species of salmon based on DNA fragments

doi: 10.1007/s00216-024-05724-9

Figure Lengend Snippet: The first line has the same sequence listed in Fig. as it was measured, and the second line has one of the 25 replicates that were generated for it based on the site-specific identification error probabilities. This particular replicate differs from the measured sequence in the seven sites marked in red

Article Snippet: We have developed a novel method to quantify the confidence surrounding the ability of a representative NextGen Sequencing method to verify the genetic identity of RM 8256, Wild-caught Coho Salmon [ ], which is a fresh frozen fish homogenate prepared from multiple individuals collected off the coast of Alaska, USA.

Techniques: Sequencing, Generated

Journal: Analytical and Bioanalytical Chemistry

Article Title: Robust discrimination between closely related species of salmon based on DNA fragments

doi: 10.1007/s00216-024-05724-9

Figure Lengend Snippet: The top section labeled “MC Replicate” has the “perturbed” sequence listed in the second line of Fig. , from one of the 16 versions of the ensemble of the high-accuracy alignments that Muscle 5.2 produces in this case. The bottom section labeled “Coho Reference Sequence” has the 654-nucleotides long reference sequence for Coho salmon. The “X” indicate “missing” sites in the replicate. The DL distance between these two sequences, disregarding all these “missing” sites, is 10

Article Snippet: We have developed a novel method to quantify the confidence surrounding the ability of a representative NextGen Sequencing method to verify the genetic identity of RM 8256, Wild-caught Coho Salmon [ ], which is a fresh frozen fish homogenate prepared from multiple individuals collected off the coast of Alaska, USA.

Techniques: Labeling, Sequencing